Sensitivity Comparison of Immuno-Histochemical and Histopathological Approaches for Diagnosis of Rabies in Animals

FAT is a gold standard and validated diagnostic test that confirm the presence of rabies virus in brain tissue. However, sophisticated laboratory infra-structure and highly trained personnel are required for conducting rabies diagnosis by immunofluorescence. On the other hand, immunohistochemistry (IHC), with its immunological approach of antigen detection is expected to be close to FAT in sensitivity of detection of rabies in brain tissues. IHC is also feasible on formalin fixed histological sections in most of the laboratories where routine histopathological approach is feasible. IHC is expected to detect rabies antigen that is not discernible with histopathological examination. Thus, the present study was envisaged to elucidate the feasibility of IHC International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 4 (2017) pp. 400-405 Journal homepage: http://www.ijcmas.com